Human SPN (Leukosialin) QuickTest ELISA Kit
SKU: 46782739448

Human SPN (Leukosialin) QuickTest ELISA Kit

Sale price$263.25 Regular price$292.50
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Description

Human SPN (Leukosialin) QuickTest ELISA KitThe Human SPN (Leukosialin) QuickTest ELISA Kit measures SPN in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into individual

The Human SPN (Leukosialin) QuickTest ELISA Kit measures SPN in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains SPN, a capture antibody-SPN-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of SPN in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 46782739448

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Reviewed in the United States on May 12, 2026
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AnneMarie H.
Lexington, US
★★★★★ 1
Not as described
Color: Colorful, Size: Large, Color: Colorful, Size: Large
This item is vibrant in color, but I could tell right away, It wasn’t going to be durable and indestructible like the description said. My dog had it destroyed in less than a minute.
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Reviewed in the United States on May 29, 2026
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Lowell, US
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Great play for everyone
Color: Colorful-2, Size: Medium, Color: Colorful-2, Size: Medium
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